| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-X-C chemokine receptor type 2; CXC-R2; CXCR-2; CDw128b; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8R B; IL-8 receptor type 2; CD antigen CD182; CXCR2; IL8RB |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR2, which shares 52.4% amino acid (aa) sequence identity with rat CXCR2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects C-X-C chemokine receptor type 2 (gene CXCR2) in human samples and is validated for IHC and flow cytometry. The predicted molecular weight is 40.8 kDa.
CXCR2 is a G protein-coupled receptor on neutrophils that binds IL-8 and several other ELR-motif CXC chemokines, including CXCL1, CXCL2, CXCL3, CXCL5 and CXCL7. It is the main driver of neutrophil migration out of the bone marrow and into inflamed or wounded tissue, and it is studied in inflammation, wound healing and cancer.
| Target | C-X-C chemokine receptor type 2 (gene CXCR2; UniProt P25025, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 40.8 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with confirmed staining (IHC): human spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): human PBMC cells.
Immunogen
Synthetic peptide from the N-terminal region of human CXCR2. Sequence identity with the rat ortholog: 52.4%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places C-X-C chemokine receptor type 2 in the cell membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
