| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Neutrophil gelatinase-associated lipocalin;NGAL;Lipocalin-2;SV-40-induced 24P3 protein;Siderocalin LCN2;p25;Lcn2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human CXCR2 recombinant protein (Position: M1-Q216). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-CXCR2 Antibody Picoband® is an antibody for CXCR2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: CXCR2 (Neutrophil gelatinase-associated lipocalin); UniProt: P25025
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 41-50 kDa, calculated 22875 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-CXCR2 Antibody Picoband® catalog # A00455-3.
Biological background
Biological context: Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. .
Expression and localization notes: cellular localization: Secreted . Upon binding to the SLC22A17 (24p3R) receptor, it is internalized., tissue context: Detected in lung, spleen, uterus, vagina and epididymis. ..
Common research applications
- Western blotting (WB): Compare CXCR2 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of CXCR2 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify CXCR2-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: CXCR2 is a receptor for Interleukin 8, which is a powerful neutrophil chemotactic factor. It is a member of the GPCR family (subfamily, chemokine). Binding of IL8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activate a phosphatidylinositol-calcium second messenger system. This receptor binds to IL8 with a high affinity and to GRO/MGSA and NAP2 also with a high affinity. It has been reported to be expressed in a wide variety of tissues. ESTs have been isolated from human placenta and thymus libraries.
- Cross reactivity: No cross-reactivity with other proteins
- Cellular localization: Secreted . Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
- Tissue details: Detected in lung, spleen, uterus, vagina and epididymis. .
- Research category: Immunoglobulins,Immunology
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
