| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-X-C chemokine receptor type 2; CXC-R2; CXCR-2; CDw128b; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8R B; IL-8 receptor type 2; CD antigen CD182; CXCR2; IL8RB |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CXCR2 recombinant protein (Position: M1-Q216). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects C-X-C chemokine receptor type 2 (gene CXCR2) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 40.8 kDa, and the supplier reports an observed band at about 41–50 kDa.
CXCR2 is a G protein-coupled receptor on neutrophils that binds IL-8 and several other ELR-motif CXC chemokines, including CXCL1, CXCL2, CXCL3, CXCL5 and CXCL7. It is the main driver of neutrophil migration out of the bone marrow and into inflamed or wounded tissue, and it is studied in inflammation, wound healing and cancer.
| Target | C-X-C chemokine receptor type 2 (gene CXCR2; UniProt P25025, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 40.8 kDa |
| Observed MW | 41–50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human COLO-320 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): THP-1 cells.
Immunogen
Recombinant human CXCR2 fragment (Met1–Gln216), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places C-X-C chemokine receptor type 2 in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liu Xiaoming et al. (2016) Spinal translocator protein alleviates chronic neuropathic pain behavior and modulates spinal astrocyte–neuronal function in rats with L5 spinal nerve ligation model. Pain. 10.1097/j.pain.0000000000000339
De-Li Cao et al. (2014) Chemokine CXCL1 enhances inflammatory pain and increases NMDA receptor activity and COX-2 expression in spinal cord neurons via activation of CXCR2. Experimental Neurology. 10.1016/j.expneurol.2014.05.014
Fengjiao Wu et al. (2024) Cerebral Endothelial CXCR2 Promotes Neutrophil Transmigration into Central Nervous System in LPS-Induced Septic Encephalopathy. Biomedicines. 10.3390/biomedicines12071536
Liu Guangchen et al. (2019) Activation of CXCL6/CXCR1/2 Axis Promotes the Growth and Metastasis of Osteosarcoma Cells in vitro and in vivo. Frontiers in Pharmacology. 10.3389/fphar.2019.00307
Dong Cui et al. (2018) Activated CXCL5-CXCR2 axis promotes the migration, invasion and EMT of papillary thyroid carcinoma cells via modulation of β-catenin pathway. Biochimie. 10.1016/j.biochi.2018.02.009
Yuan Zhou et al. (2016) Overexpression of GRK6 attenuates neuropathic pain via suppression of CXCR2 in rat dorsal root ganglion. Molecular Pain. 10.1177/1744806916646381
De-Li Cao et al. (2016) Chemokine receptor CXCR2 in dorsal root ganglion contributes to the maintenance of inflammatory pain. Brain Research Bulletin. 10.1016/j.brainresbull.2016.09.016
