| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-X-C chemokine receptor type 3; CXC-R3; CXCR-3; CKR-L2; G protein-coupled receptor 9; Interferon-inducible protein 10 receptor; IP-10 receptor; CD antigen CD183; CXCR3; GPR9 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CXCR3 Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC) . Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC) . May play a role in angiogenesis. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACFD-3 is a rabbit monoclonal antibody that recognizes C-X-C chemokine receptor type 3 (gene CXCR3) in human samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 40.7 kDa, and the supplier reports an observed band at about 41 kDa.
CXCR3 is a G protein-coupled chemokine receptor for CXCL9, CXCL10 and CXCL11. It is expressed on activated T cells, especially Th1 and CD8+ effector cells, and on NK cells, and guides them into inflamed tissues and tumors. Alternative isoforms also bind CXCL4. It is studied in Th1 immunity, autoimmunity, transplantation and tumor immunology.
| Target | C-X-C chemokine receptor type 3 (gene CXCR3; UniProt P49682, human) |
|---|---|
| Clone | ACFD-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 40.7 kDa |
| Observed MW | 41 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human CXCR3 Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC) . Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC) . May play a role in angiogenesis.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
