| Field | Specification |
|---|---|
| Target | |
| Alternative names | C-X-C chemokine receptor type 5; CXC-R5; CXCR-5; Burkitt lymphoma receptor 1 homolog; CD antigen CD185; Cxcr5; Blr1; Gpcr6 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Cxcr5 recombinant protein (Position: M1-F374). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects C-X-C chemokine receptor type 5 (gene Cxcr5) in mouse and rat samples and is validated for IHC and ELISA. The predicted molecular weight is 42.1 kDa.
CXCR5 is a G protein-coupled receptor for the chemokine CXCL13. It is expressed on B cells and follicular helper T cells and directs them into B-cell follicles of lymphoid organs, where germinal centers form. CXCR5 is a standard marker of follicular helper T cells in studies of antibody responses and lymphoid organization.
| Target | C-X-C chemokine receptor type 5 (gene Cxcr5; UniProt Q04683, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 42.1 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with confirmed staining (IHC): mouse spleen tissue, rat lymph nodes tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse Cxcr5 fragment (Met1–Phe374), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places C-X-C chemokine receptor type 5 in the cell membrane. Tissue expression noted by UniProt (mouse): Mainly in spleen, in resting B-cells.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
