| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclin-A1;CCNA1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Cyclin A1 recombinant protein (Position: D64-E263). Human Cyclin A1 shares 78.5% and 79.5% amino acid (aa) sequence identity with mouse and rat Cyclin A1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects cyclin-A1 (gene CCNA1) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF and Western blot. The predicted molecular weight is 52.4 kDa, and the supplier reports an observed band at about 52 kDa.
Cyclin A1 (CCNA1) is an A-type cyclin expressed mainly in the germline, especially in male meiotic cells. It partners with cyclin-dependent kinases to control cell cycle transitions and is required for progression through meiosis in spermatogenesis, so it is studied in reproductive biology and in some leukemias where it is aberrantly expressed.
| Target | Cyclin-A1 (gene CCNA1; UniProt P78396, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 52.4 kDa |
| Observed MW | 52 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human 293T cells, human MCF-7 cells, human hela whole cellrat kidney tissue, rat testis tissue, mouse kidney tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A549 cells.
Samples with a confirmed signal (flow cytometry): PC-3 cells.
Immunogen
Recombinant human Cyclin A1 fragment (Asp64–Glu263), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 78.5% and 79.5%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cyclin-A1 in the nucleus. Tissue expression noted by UniProt (human): Very high levels in testis and very low levels in brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ma Cui et al. (2026) Super enhancer-driven LINC01013 mediates hypoxia-induced mitochondrial dysfunction by HSPA9 to determine pulmonary arterial smooth muscle cell fate. Cellular and Molecular Life Sciences. 10.1007/s00018-025-06071-3
Shuang Li et al. (2024) Network pharmacology-based investigation of the effects of Shenqi Fuzheng injection on glioma proliferation and migration via the SRC/PI3K/AKT signaling pathway. Journal of Ethnopharmacology. 10.1016/j.jep.2024.118128
Yuan Yuncang et al. (2016) Overexpression of miR-30a in lung adenocarcinoma A549 cell line inhibits migration and invasion via targeting EYA2. ACTA Biochimica et Biophysica Sinica. 10.1093/abbs/gmv139
Lin Ping et al. (2010) Fbxw8 is involved in the proliferation of human choriocarcinoma JEG-3 cells. Molecular Biology Reports. 10.1007/s11033-010-0288-7
Zhang Lixin et al. (2023) Paeonol Represses A549 Cell Glycolytic Reprogramming and Proliferation by Decreasing m6A Modification of Acyl-CoA Dehydrogenase. Chinese Journal of Physiology. 10.4103/cjop.CJOP-D-22-00166
Jie Li et al. (2006) Specific COX-2 inhibitor, meloxicam, suppresses proliferation and induces apoptosis in human HepG2 hepatocellular carcinoma cells. Journal of Gastroenterology and Hepatology. 10.1111/j.1440-1746.2006.04366.x
Miao Ying et al. (2009) Growth inhibition and cell-cycle arrest of human gastric cancer cells by Lycium barbarum polysaccharide. Medical Oncology. 10.1007/s12032-009-9286-9
Wang Liang et al. (2018) IL-2Rα up-regulation is mediated by latent membrane protein 1 and promotes lymphomagenesis and chemotherapy resistance in natural killer/T-cell lymphoma. Cancer Communications. 10.1186/s40880-018-0334-8
