| Field | Specification |
|---|---|
| Target | |
| Alternative names | G2/mitotic-specific cyclin-B1;CCNB1;CCNB; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Cyclin B1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EOG-3 is a rabbit monoclonal antibody that recognizes G2/mitotic-specific cyclin-B1 (gene CCNB1) in human and mouse samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 48.3 kDa, and the supplier reports an observed band at about 55 kDa.
Cyclin B1 (CCNB1) binds and activates CDK1 to form maturation-promoting factor, the kinase that triggers entry into mitosis. Its levels rise through G2 and it is destroyed at the metaphase-to-anaphase transition, so it is a standard marker of G2/M cells in cell cycle and cancer studies.
| Target | G2/mitotic-specific cyclin-B1 (gene CCNB1; UniProt P14635, human) |
|---|---|
| Clone | EOG-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 48.3 kDa |
| Observed MW | 55 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:100 |
Samples with confirmed staining (IHC): human colon tissue, human lung large cells cancer tissue, rat stomach tissue, mouse lung tissue, human esophageal carcinoma tissue, human colon cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human cyclin B1.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places G2/mitotic-specific cyclin-B1 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xin Zhou et al. (2023) Transcriptomics and Molecular Docking Reveal the Potential Mechanism of Lycorine Against Pancreatic Cancer. Phytomedicine. 10.1016/j.phymed.2023.155128
Juan Chen et al. (2021) PKMYT1, exacerbating the progression of clear cell renal cell carcinoma, is implied as a biomarker for the diagnosis and prognosis. Aging-US. 10.18632/aging.203759
Huihui Ma et al. (2024) Alkannin Induces G2/M-Phase Arrest, Apoptosis, and Inhibition of Invasion by Targeting GSK3β in Esophageal Squamous Cell Carcinoma. Drug Design Development and Therapy. 10.2147/DDDT.S470061
Yanli Guo et al. (2024) Bufadienolides from Chansu Injection Synergistically Enhances the Antitumor Effect of Erlotinib by Inhibiting the KRAS Pathway in Pancreatic Cancer. Pharmaceuticals. 10.3390/ph17121696
