| Field | Specification |
|---|---|
| Target | |
| Alternative names | G1/S-specific cyclin-D1;B-cell lymphoma 1 protein;BCL-1;BCL-1 oncogene;PRAD1 oncogene;CCND1;BCL1, PRAD1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Cyclin D1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DAD-3 is a rabbit monoclonal antibody that recognizes G1/S-specific cyclin-D1 (gene CCND1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 33.7 kDa, and the supplier reports an observed band at about 34 kDa.
Cyclin D1 (CCND1) pairs with CDK4 or CDK6 to phosphorylate the retinoblastoma protein RB1 in G1, releasing E2F transcription factors and allowing cells to enter S phase. Its expression responds to mitogenic signals, and it is frequently amplified or overexpressed in cancers such as breast cancer and mantle cell lymphoma.
| Target | G1/S-specific cyclin-D1 (gene CCND1; UniProt P24385, human) |
|---|---|
| Clone | DAD-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 33.7 kDa |
| Observed MW | 34 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human MCF-7 cells, human LNCAP cells, human HepG2 cells, human A431 cells, rat brain tissue, rat liver tissue, mouse brain tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Samples with confirmed staining (IHC): human bladder tissue, human pituitary tumor tissue, human squamous cells carcinoma tissue, human ovarian cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human cyclin D1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places G1/S-specific cyclin-D1 in the nucleus, cytoplasm and nuclear membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Shen Shengping et al. (2024) MAT1A activation of glycolysis to promote NSCLC progression depends on stabilizing CCND1. Cell Death & Disease. 10.1038/s41419-024-07113-7
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Mengyu Sun et al. (2022) Sdy-1 Executes Antitumor Activity in HepG2 and HeLa Cancer Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway. Marine Drugs. 10.3390/md20020125
Xue Bai et al. (2022) Nuciferine Inhibits TMEM16A in Dietary Adjuvant Therapy for Lung Cancer. Journal of Agricultural and Food Chemistry. 10.1021/acs.jafc.1c08375
Juanjuan Han et al. (2023) Andrographolide inhibits the proliferation and migration of vascular smooth muscle cells via PI3K/AKT signaling pathway and amino acid metabolism to prevent intimal hyperplasia. European Journal of Pharmacology. 10.1016/j.ejphar.2023.176082
Xian Zhang et al. (2022) SIRT6 Promotes the Progression of Prostate Cancer via Regulating the Wnt/β-Catenin Signaling Pathway. Journal of Oncology. 10.1155/2022/2174758
