| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclin-dependent kinase 2;2.7.11.22;Cell division protein kinase 2;p33 protein kinase;CDK2;CDKN2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Cdk2, identical to the related rat and mouse sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes cyclin-dependent kinase 2 (gene CDK2) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 33.9 kDa, and the supplier reports an observed band at about 30 kDa.
CDK2 is a cyclin-dependent kinase that pairs with cyclin E and cyclin A to drive the G1-to-S transition and DNA replication. It phosphorylates targets such as RB1 and helps trigger centrosome duplication; although somatic cells can often compensate for its loss, it is essential for meiosis and is widely studied in cell cycle research.
| Target | Cyclin-dependent kinase 2 (gene CDK2; UniProt P24941, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 33.9 kDa |
| Observed MW | 30 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human Jurkat cells lysate, human HepG2 cells lysate, human U2OS cells lysate, human K562 cells lysate, human T47D cells lysate, human Caco-2 cells lysate, human 293T cells lysate, human PC-3 cells lysate, rat L6 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human cdk2. The peptide sequence is conserved in rat and mouse.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cyclin-dependent kinase 2 in the nucleus, cytoplasm and endosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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