| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclin-dependent kinase 7;2.7.11.22;2.7.11.23;39 kDa protein kinase;p39 Mo15;CDK-activating kinase 1;Cell division protein kinase 7;Serine/threonine-protein kinase 1;TFIIH basal transcription factor complex kinase subunit;CDK7;CAK, CAK1, CDKN7, MO15, STK1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Cdk7, different from the related rat and mouse sequences by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects cyclin-dependent kinase 7 (gene CDK7) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 39.0 kDa, and the supplier reports an observed band at about 100 kDa.
CDK7 is the kinase at the core of the CDK-activating kinase (CAK), which it forms with cyclin H and MAT1. It activates other CDKs such as CDK1 and CDK2 by phosphorylating their T-loop, and as part of the general transcription factor TFIIH it phosphorylates RNA polymerase II, linking cell cycle control to transcription.
| Target | Cyclin-dependent kinase 7 (gene CDK7; UniProt P50613, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 39.0 kDa |
| Observed MW | 100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat, Mouse) |
|---|
Samples with a confirmed band (WB): rat testis tissue, rat ovary tissue, HeLa cells, MCF-7 cells, A549 cells, COLO320 cells, Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the C-terminal region of human cdk7. It differs from the rat and mouse sequences at two residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cyclin-dependent kinase 7 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
