| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | G1/S-specific cyclin-E1; CCNE1; CCNE |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Cyclin E1/CCNE1 recombinant protein (Position: R3-A386). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Cyclin E1/CCNE1 Antibody Picoband® is an antibody for CCNE1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: CCNE1 (cyclin E1); UniProt: P24864
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 47 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Cyclin E1/CCNE1 Antibody Picoband® catalog # A00543-2.
Biological background
Biological context: Essential for the control of the cell cycle at the G1/S (start) transition.
Expression and localization notes: cellular localization: Nucleus., tissue context: Highly expressed in testis and placenta. Low levels in bronchial epithelial cells..
Common research applications
- Western blotting (WB): Compare CCNE1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of CCNE1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify CCNE1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: G1/S-specific cyclin-E1 is a protein that in humans is encoded by the CCNE1 gene. It is mapped to 19q12. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleus.
- Tissue details: Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
- Research category: Angiogenesis,Cancer,Cancer Metabolism,Cardiovascular,Growth Factors,Invasion/Microenvironment,Metabolism,Metabolism Processes,Pathways and Processes,Protein Phosphorylation,Response To Hypoxia,Signal Transduction,Tyrosine Kinases
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
