| Field | Specification |
|---|---|
| Target | |
| Alternative names | G1/S-specific cyclin-E1;CCNE1;CCNE; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Cyclin E1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DDF-3 is a rabbit monoclonal antibody that recognizes G1/S-specific cyclin-E1 (gene CCNE1) in human samples and is validated for Western blot, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 47.1 kDa, and the supplier reports an observed band at about 49 kDa.
Cyclin E1 (CCNE1) activates CDK2 in late G1 to promote the transition into S phase and the start of DNA replication. It is tightly regulated by ubiquitin-mediated degradation, and CCNE1 amplification is well documented in several cancers, notably high-grade serous ovarian cancer.
| Target | G1/S-specific cyclin-E1 (gene CCNE1; UniProt P24864, human) |
|---|---|
| Clone | DDF-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 47.1 kDa |
| Observed MW | 49 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human cyclin E1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places G1/S-specific cyclin-E1 in the nucleus. Tissue expression noted by UniProt (human): Highly expressed in testis and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
