| Field | Specification |
|---|---|
| Target | |
| Alternative names | G1/S-specific cyclin-E2;CCNE2; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Cyclin E2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EDB-3 is a rabbit monoclonal antibody that recognizes G1/S-specific cyclin-E2 (gene CCNE2) in human samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 46.8 kDa; the supplier reports observed bands at 47, 50 kDa.
Cyclin E2 (CCNE2) is an E-type cyclin that, like cyclin E1, binds and activates CDK2 during late G1 and early S phase. It helps commit cells to DNA replication and is studied in cell cycle control and in tumors where E-type cyclins are overexpressed.
| Target | G1/S-specific cyclin-E2 (gene CCNE2; UniProt O96020, human) |
|---|---|
| Clone | EDB-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 46.8 kDa |
| Observed MW | 47, 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human cyclin E2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places G1/S-specific cyclin-E2 in the nucleus. Tissue expression noted by UniProt (human): According to PubMed:9858585, highest levels of expression in adult testis, thymus and brain.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
