| Field | Specification |
|---|---|
| Target | |
| Alternative names | G1/S-specific cyclin-E2;CCNE2; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Cyclin E2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GHH-3 is a rabbit monoclonal antibody that recognizes G1/S-specific cyclin-E2 (gene CCNE2) in human and mouse samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 46.8 kDa, and the supplier reports an observed band at about 47 kDa.
Cyclin E2 (CCNE2) is an E-type cyclin that, like cyclin E1, binds and activates CDK2 during late G1 and early S phase. It helps commit cells to DNA replication and is studied in cell cycle control and in tumors where E-type cyclins are overexpressed.
| Target | G1/S-specific cyclin-E2 (gene CCNE2; UniProt O96020, human) |
|---|---|
| Clone | GHH-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 46.8 kDa |
| Observed MW | 47 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human breast carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human cyclin E2.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places G1/S-specific cyclin-E2 in the nucleus. Tissue expression noted by UniProt (human): According to PubMed:9858585, highest levels of expression in adult testis, thymus and brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hanliang Sun et al. (2025) 5-Methylcytosine-modified circRNA-CCNL2 regulates vascular remdeling in hypoxic pulmonary hypertension through binding to FXR2. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2025.139638
Xu Li-Xiao et al. (2014) Histone acetyltransferase inhibitor II induces apoptosis in glioma cell lines via the p53 signaling pathway. Journal of Experimental & Clinical Cancer Research. 10.1186/s13046-014-0108-3
Haolong Zhang et al. (2018) Wall shear stress promotes intimal hyperplasia through the paracrine H2O2-mediated NOX-AKT-SVV axis. Life Sciences. 10.1016/j.lfs.2018.05.045
Hui Liu et al. (2017) Down-regulation of long non-coding RNA MALAT1 by RNA interference inhibits proliferation and induces apoptosis in multiple myeloma. Clinical and Experimental Pharmacology and Physiology. 10.1111/1440-1681.12804
Siqi Wang et al. (2019) Downregulation of long non‑coding RNA ANRIL promotes proliferation and migration in hypoxic human pulmonary artery smooth muscle cells. Molecular Medicine Reports. 10.3892/mmr.2019.10887
