| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytoplasmic FMR1-interacting protein 1; Specifically Rac1-associated protein 1; Sra-1; p140sra-1; CYFIP1; KIAA0068 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CYFIP1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29C25 is a rabbit monoclonal antibody that recognizes cytoplasmic FMR1-interacting protein 1 (gene CYFIP1) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 145.2 kDa, and the supplier reports an observed band at about 145 kDa.
CYFIP1 is a large adaptor protein with two known roles: it is a core subunit of the WAVE regulatory complex, which activates Arp2/3-driven actin assembly downstream of RAC1, and it binds FMRP and eIF4E to repress translation of specific mRNAs in neurons. It is studied in synapse development, dendritic spine morphology and neurodevelopmental disorders.
| Target | Cytoplasmic FMR1-interacting protein 1 (gene CYFIP1; UniProt Q7L576, human) |
|---|---|
| Clone | 29C25 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 145.2 kDa |
| Observed MW | 145 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human SH-SY5Y cells, human U2OS cells, human U251 cells, rat brain tissue, rat C6 cells, mouse brain tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human CYFIP1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cytoplasmic FMR1-interacting protein 1 in the cytoplasm, cell projection and synapse. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
