| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sterol 26-hydroxylase, mitochondrial; 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 26-hydroxylase; Cytochrome P-450C27/25; Cytochrome P450 27; Sterol 27-hydroxylase; Vitamin D(3) 25-hydroxylase; CYP27A1; CYP27 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human CYP27A1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20C32 is a rabbit monoclonal antibody that recognizes sterol 26-hydroxylase, mitochondrial (gene CYP27A1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 60.2 kDa; the supplier reports observed bands at 50, 60 kDa.
Sterol 27-hydroxylase (CYP27A1) is a mitochondrial cytochrome P450 that oxidizes the side chain of cholesterol and related sterols. It participates in bile acid synthesis, especially via the alternative (acidic) pathway, and forms 27-hydroxycholesterol, an oxysterol signal. Loss of CYP27A1 causes cerebrotendinous xanthomatosis, and it is studied in cholesterol and bile acid metabolism.
| Target | Sterol 26-hydroxylase, mitochondrial (gene CYP27A1; UniProt Q02318, human) |
|---|---|
| Clone | 20C32 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 60.2 kDa |
| Observed MW | 50, 60 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:80 |
Samples with a confirmed band (WB): human HepG2 cells, human Caco-2 cells, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human CYP27A1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sterol 26-hydroxylase, mitochondrial in the mitochondrion inner membrane. Tissue expression noted by UniProt (human): Expressed in the neural retina and underlying retinal pigment epithelium (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
