| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cystatin-C; Cystatin-3; Cst3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse Cystatin C recombinant protein (Position: A21-A140). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets cystatin-C (gene Cst3) in mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 15.5 kDa, and the supplier reports an observed band at about 16 kDa.
Cystatin C (CST3) is a small secreted inhibitor of cysteine proteases, including cathepsins, made by nearly all nucleated cells. Because it is produced at a steady rate and cleared by glomerular filtration, its level in blood is widely used to estimate kidney function, and it is also studied in vascular and neurodegenerative disease.
| Target | Cystatin-C (gene Cst3; UniProt P21460, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 15.5 kDa |
| Observed MW | 16 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA (capture antibody) | 1–5 µg/mL |
Samples with a confirmed band (WB): mouse brain tissue, mouse spleen tissue, mouse heart tissue, mouse lung tissue, mouse kidney tissue, mouse testis tissue, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant mouse Cystatin C fragment (Ala21–Ala140), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes cystatin-C as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Kai-Di Ren et al. (2019) Mitochondrial E3 ubiquitin ligase 1 promotes brain injury by disturbing mitochondrial dynamics in a rat model of ischemic stroke. European Journal of Pharmacology. 10.1016/j.ejphar.2019.172617
