| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cystatin-C;Cystatin-3;Gamma-trace;Neuroendocrine basic polypeptide;Post-gamma-globulin;CST3; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Cystatin C |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IEE-3 is a rabbit monoclonal antibody that recognizes cystatin-C (gene CST3) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 15.8 kDa, and the supplier reports an observed band at about 16 kDa.
Cystatin C (CST3) is a small secreted inhibitor of cysteine proteases, including cathepsins, made by nearly all nucleated cells. Because it is produced at a steady rate and cleared by glomerular filtration, its level in blood is widely used to estimate kidney function, and it is also studied in vascular and neurodegenerative disease.
| Target | Cystatin-C (gene CST3; UniProt P01034, human) |
|---|---|
| Clone | IEE-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 15.8 kDa |
| Observed MW | 16 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells, rat brain tissue, rat testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human cystatin C.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes cystatin-C as a secreted protein. Tissue expression noted by UniProt (human): Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yanling Tang et al. (2023) PRKAA1 induces aberrant mitophagy in a PINK1/Parkin-dependent manner, contributing to fluoride-induced developmental neurotoxicity. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2023.114772
Ying Zhu et al. (2019) Protective effects of mild hypothermia against hepatic injury in rats with acute liver failure. Annals of Hepatology. 10.1016/j.aohep.2019.01.008
