Anti-Cystatin C CST3 Rabbit Monoclonal Antibody

Overview

This product is an anti-CST3 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone IEE-3; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC (as provided in the source record). Boster Bio Anti-Cystatin C CST3 Rabbit Monoclonal Antibody catalog # M00961. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: CST3 (Cystatin-C).
• Antibody format: Monoclonal; clone IEE-3; isotype Rabbit IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

CST3 (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Reported cellular localization context: Secreted . Tissue expression notes (as provided): Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. .

Research relevance and current trends

• Research context keywords from the source record include: Apoptosis,Atherosclerosis,Cardiovascular,Caspases,Cell Biology,Cell Type Markers,Intracellular,Neural Stem Cells,Stem Cells,Tags & Cell Markers,Tumor Associated.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.

Workflow ideas (metafield): Validate CST3 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect CST3 expression by Western blot in cell or tissue lysates, Detect CST3 in FFPE tissue sections by immunohistochemistry

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 16 kDa; calculated MW: 15799 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 16 kDa
• Cellular localization (provided): Secreted .
• Tissue details (provided): Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. .

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.