Anti-Cyt 19/As3mt Antibody Picoband®

Overview

Anti-Cyt 19/As3mt Antibody Picoband® is an antibody for As3mt detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA, IP workflows.

Key elements and design rationale

• Target: As3mt (SUMO specific peptidase 6); UniProt: Q91WU5
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 42-45 kDa, calculated 42 kDa
• Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA, IP

Vendor description (summary): Boster Bio Anti-Cyt 19/As3mt Antibody Picoband® catalog # A05107-2.

Biological background

Biological context: Protease that deconjugates SUMO1, SUMO2 and SUMO3 from targeted proteins. Processes preferentially poly-SUMO2 and poly-SUMO3 chains, but does not efficiently process SUMO1, SUMO2 and SUMO3 precursors. Deconjugates SUMO1 from RXRA, leading to transcriptional activation. Involved in chromosome alignment and spindle assembly, by regulating the kinetochore CENPH-CENPI-CENPK complex. Desumoylates PML and CENPI, protecting them from degradation by the ubiquitin ligase RNF4, which targets polysumoylated proteins for proteasomal degradation. Desumoylates also RPA1, thus preventing recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination.

Expression and localization notes: cellular localization: Nucleus., tissue context: Expressed in many tissues, highest levels in skeletal muscle..

Common research applications

• Western blotting (WB): Compare As3mt levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of As3mt in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify As3mt-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: AS3MT catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to trivalent arsenical and may play a role in arsenic metabolism. This gene is mapped to chromosome 10q24.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Nucleus.
• Tissue details: Expressed in many tissues, highest levels in skeletal muscle.
• Research category: Cancer,Lipid Metabolism,Metabolism,Signal Transduction

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.