| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytochrome c;CYCS;CYC; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Cytochrome C recombinant protein (Position: G2-E105). Human Cytochrome C shares 91% amino acid (aa) sequence identity with both mouse and rat Cytochrome C. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets cytochrome c (gene CYCS) in human, mouse and rat samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 11.7 kDa, and the supplier reports an observed band at about 14 kDa.
Cytochrome c (CYCS) is a small heme protein in the mitochondrial intermembrane space that shuttles electrons from complex III to complex IV in the respiratory chain. When the outer mitochondrial membrane is permeabilized during apoptosis, it is released into the cytosol, binds APAF1 and triggers the apoptosome and caspase-9 activation, so it is a classic marker of intrinsic apoptosis.
| Target | Cytochrome c (gene CYCS; UniProt P99999, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 11.7 kDa |
| Observed MW | 14 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, human HepG2 cells, rat kidney tissue, rat skeletal muscle tissue, mouse kidney tissue, mouse skeletal muscle tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse brain tissue, rat brain tissue, human intestinal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): SMMC-7721 cells.
Samples with confirmed staining (ICC/IF): human intestinal cancer tissue, mouse cardiac muscle tissue, rat cardiac muscle tissue.
Immunogen
Recombinant human Cytochrome C fragment (Gly2–Glu105), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 91%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cytochrome c in the mitochondrion intermembrane space. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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