| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytochrome c, somatic;Cycs; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse Cytochrome C recombinant protein (Position: G2-E105). Mouse Cytochrome C shares 91.3% and 100% amino acid (aa) sequence identity with human and rat Cytochrome C, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes cytochrome c, somatic (gene Cycs) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 11.6 kDa, and the supplier reports an observed band at about 14 kDa.
Cytochrome c (CYCS) is a small heme protein in the mitochondrial intermembrane space that shuttles electrons from complex III to complex IV in the respiratory chain. When the outer mitochondrial membrane is permeabilized during apoptosis, it is released into the cytosol, binds APAF1 and triggers the apoptosome and caspase-9 activation, so it is a classic marker of intrinsic apoptosis.
| Target | Cytochrome c, somatic (gene Cycs; UniProt P62897, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 11.6 kDa |
| Observed MW | 14 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human HL-60 cells, human K562 cells, human U-937 cells, human U2OS cells, human T-47D cells, human PC-3 cells, human Caco-2 cells, rat brain tissue, rat heart tissue, rat kidney tissue, rat C6 cells, mouse brain tissue, mouse heart tissue, mouse kidney tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse brain tissue, mouse intestine tissue, rat brain tissue, rat intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse Cytochrome C fragment (Gly2–Glu105), expressed in E. coli. Sequence identity with the human and rat orthologs: 91.3% and 100%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cytochrome c, somatic in the mitochondrion intermembrane space. Tissue expression noted by UniProt (mouse): Found in embryos and in adult liver and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yan Li et al. (2024) Chondroitin sulfate-modified antiangiogenic peptide conjugate induces cell apoptosis via the mitochondria-mediated pathway to perform antitumor activity. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2024.129671
Yang Fang et al. (2025) Smart biodegradable hydrogels incorporating a photoactive Ir(III) complex for NIR-triggered oral cancer treatment. Cancer Nanotechnology. 10.1186/s12645-025-00336-z
Junbo Li et al. (2023) Polystyrene microplastics mediate inflammatory responses in the chicken thymus by Nrf2/NF-κB pathway and trigger autophagy and apoptosis. Environmental Toxicology and Pharmacology. 10.1016/j.etap.2023.104136
Tiantian Guo et al. (2024) New insights into the spleen injury by mitochondrial dysfunction of chicken under polystyrene microplastics stress. Poultry Science. 10.1016/j.psj.2024.103674
Xiaobo Wang et al. (2019) Rhodiola crenulata attenuates apoptosis and mitochondrial energy metabolism disorder in rats with hypobaric hypoxia-induced brain injury by regulating the HIF-1α/microRNA 210/ISCU1/2(COX10) signaling pathway. Journal of Ethnopharmacology. 10.1016/j.jep.2019.03.028
Xiang Sen et al. (2019) Mitochondria-Targeted and Resveratrol-Loaded Dual-Function Titanium Disulfide Nanosheets for Photothermal-Triggered Tumor Chemotherapy. Nanoscale Research Letters. 10.1186/s11671-019-3044-5
Keisuke Imoto et al. (2018) Canstatin modulates L-type calcium channel activity in rat ventricular cardiomyocytes. Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2018.04.026
Zhen Zhang et al. (2019) Astragaloside IV alleviates myocardial damage induced by type 2 diabetes via improving energy metabolism. Molecular Medicine Reports. 10.3892/mmr.2019.10716
Ya Hou et al. (2019) Establishment and evaluation of a simulated high‑altitude hypoxic brain injury model in SD rats. Molecular Medicine Reports. 10.3892/mmr.2019.9939
Wang Han et al. (2024) Adiponectin attenuates H2O2-induced apoptosis in chicken skeletal myoblasts through the lysosomal-mitochondrial axis. In Vitro Cellular & Developmental Biology-Animal. 10.1007/s11626-024-00857-8
