| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytochrome c;CYCS;CYC; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Cytochrome C |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GOC-3 is a rabbit monoclonal antibody that recognizes cytochrome c (gene CYCS) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 11.7 kDa, and the supplier reports an observed band at about 14 kDa.
Cytochrome c (CYCS) is a small heme protein in the mitochondrial intermembrane space that shuttles electrons from complex III to complex IV in the respiratory chain. When the outer mitochondrial membrane is permeabilized during apoptosis, it is released into the cytosol, binds APAF1 and triggers the apoptosome and caspase-9 activation, so it is a classic marker of intrinsic apoptosis.
| Target | Cytochrome c (gene CYCS; UniProt P99999, human) |
|---|---|
| Clone | GOC-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 11.7 kDa |
| Observed MW | 14 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:30 |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, human HepG2 cells, human HT-1080 cells, rat kidney tissue, rat skeletal muscle tissue, mouse kidney tissue, mouse skeletal muscle tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human cytochrome C.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cytochrome c in the mitochondrion intermembrane space. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wen Tang et al. (2025) Redox-sensitive hyaluronic acid-based glycopeptide nanoparticles: Dual-action platform for mitochondrial disruption and tumor suppression. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2025.148544
Yan Li et al. (2024) Chondroitin sulfate-modified antiangiogenic peptide conjugate induces cell apoptosis via the mitochondria-mediated pathway to perform antitumor activity. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2024.129671
Shuli Man et al. (2025) Self-assembled formosanin C exerts anti-liver cancer effects by targeting STAT3 in combination with sorafenib. Colloids and Surfaces a-Physicochemical and Engineering Aspects. 10.1016/j.colsurfa.2025.139309
Wang Han et al. (2024) Adiponectin attenuates H2O2-induced apoptosis in chicken skeletal myoblasts through the lysosomal-mitochondrial axis. In Vitro Cellular & Developmental Biology-Animal. 10.1007/s11626-024-00857-8
