| Field | Specification |
|---|---|
| Target | |
| Alternative names | Steroid 17-alpha-hydroxylase/17,20 lyase |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse Cyp17a1 recombinant protein (Position: Q80-R363). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets steroid 17-alpha-hydroxylase/17,20 lyase (gene Cyp17a1) in mouse and rat samples and is validated for ELISA, ICC/IF, IHC and Western blot. The predicted molecular weight is 57.6 kDa, and the supplier reports an observed band at about 57 kDa.
CYP17A1 is a cytochrome P450 of the endoplasmic reticulum with two activities: 17α-hydroxylase, needed for cortisol synthesis, and 17,20-lyase, needed for androgen synthesis. It sits at a branch point of adrenal and gonadal steroidogenesis and is the target of the prostate cancer drug abiraterone, so it is widely studied in endocrine and cancer research.
| Target | Steroid 17-alpha-hydroxylase/17,20 lyase (gene Cyp17a1; UniProt P27786, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 57.6 kDa |
| Observed MW | 57 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat testis tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse testis tissue, rat testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): mouse testis tissue, rat testis tissue.
Immunogen
Recombinant mouse Cyp17a1 fragment (Gln80–Arg363), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places steroid 17-alpha-hydroxylase/17,20 lyase in the endoplasmic reticulum membrane and microsome membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Sili Guo et al. (2025) Semaglutide Alleviates Ovarian Oxidative Stress and Autophagy via the PI3K/AKT/mTOR Pathway in Mice with Polycystic Ovary Syndrome. Drug Design Development and Therapy. 10.2147/DDDT.S522730
Hai-Tao Gao et al. (2017) Effects of six priority controlled phthalate esters with long-term low-dose integrated exposure on male reproductive toxicity in rats. Food and Chemical Toxicology. 10.1016/j.fct.2017.01.011
Xi Wang et al. (2026) Neuregulin 4 improves white adipose tissue inflammation in mice with polycystic ovary syndrome. Peptides. 10.1016/j.peptides.2026.171496
Liu Cong et al. (2013) Endogenous release of female hormones from co-microencapsulated rat granulosa and theca cells. Biomedical Microdevices. 10.1007/s10544-013-9824-2
W. Sun et al. (2019) CYP17 gene plays a key role in goose genital growth by influencing the testosterone level at puberty. Poultry Science. 10.3382/ps/pey009
