| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tyrosine-protein phosphatase non-receptor type 1;3.1.3.48;Protein-tyrosine phosphatase 1B;PTP-1B;PTPN1;PTP1B; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Cytochrome P450 17A1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-CYP17A1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 20C02; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-Cytochrome P450 17A1 Rabbit Monoclonal Antibody catalog # M00615-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: CYP17A1 (Tyrosine-protein phosphatase non-receptor type 1).
- Antibody format: Monoclonal; clone 20C02; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
CYP17A1 (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET. . Reported cellular localization context: Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Interacts with EPHA3 at the cell membrane. Tissue expression notes (as provided): Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum. .
Research relevance and current trends
- Research context keywords from the source record include: Protein Phosphorylation,Signal Transduction,Tyrosine Phosphatases.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate CYP17A1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect CYP17A1 expression by Western blot in cell or tissue lysates, Localize CYP17A1 by immunofluorescence/immunocytochemistry in cultured cells, Quantify CYP17A1-positive cells by flow cytometry in single-cell suspensions, Enrich CYP17A1 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 32-42 kDa, 65 kDa, 75 kDa; calculated MW: 49967 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 32-42 kDa, 65 kDa, 75 kDa
- Cellular localization (provided): Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Interacts with EPHA3 at the cell membrane.
- Tissue details (provided): Highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. Overexpressed in a number of carcinomas. Also expressed in Barrett's esophagus epithelium and in the proximal duodenum. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
