| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytochrome P450 2E1;1.14.13.-;4-nitrophenol 2-hydroxylase;1.14.13.n7;CYPIIE1;Cytochrome P450-J;Cytochrome P450 2E1, N-terminally processed;CYP2E1;CYP2E; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human CYP2E1 recombinant protein (Position: M1-Y310). Human CYP2E1 shares 73% and 74% amino acid (aa) sequences identity with mouse and rat CYP2E1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets cytochrome P450 2E1 (gene CYP2E1) in mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 56.8 kDa, and the supplier reports an observed band at about 55 kDa.
CYP2E1 is a liver cytochrome P450 enzyme that oxidizes small molecules such as ethanol, acetone and acetaminophen, as well as fatty acids. It is induced by alcohol and fasting and can produce reactive oxygen species and toxic metabolites, including the acetaminophen metabolite NAPQI. It is studied in alcoholic liver injury, drug toxicity and oxidative stress.
| Target | Cytochrome P450 2E1 (gene CYP2E1; UniProt P05181, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 56.8 kDa |
| Observed MW | 55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat, Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat, Human) |
Samples with a confirmed band (WB): rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse liver tissue, rat liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human CYP2E1 fragment (Met1–Tyr310), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 73% and 74%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places cytochrome P450 2E1 in the endoplasmic reticulum membrane, microsome membrane and mitochondrion inner membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yayun Liu et al. (2019) Silibinin ameliorates hepatic lipid accumulation and oxidative stress in mice with non-alcoholic steatohepatitis by regulating CFLAR-JNK pathway. Acta Pharmaceutica Sinica B. 10.1016/j.apsb.2019.02.006
Ningning Chen et al. (2023) Lysine β-hydroxybutyrylation promotes lipid accumulation in alcoholic liver disease. Biochemical Pharmacology. 10.1016/j.bcp.2023.115936
Li Xin-Xin et al. (2018) Hepatoprotective effect of gastrodin against alcohol-induced liver injury in mice. Journal of Physiology and Biochemistry. 10.1007/s13105-018-0647-8
Tan Juan et al. (2019) Hepatoprotective effect of essential oils of Nepeta cataria L. on acetaminophen-induced liver dysfunction. Bioscience Reports. 10.1042/BSR20190697
