| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytoglobin;Histoglobin;HGb;Stellate cell activation-associated protein;CYGB;STAP; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Cytoglobin, identical to the related rat sequence, different from the related mouse sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes cytoglobin (gene CYGB) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 21.4 kDa, and the supplier reports an observed band at about 21 kDa.
Cytoglobin (CYGB) is a vertebrate globin with a hexacoordinated heme, expressed mainly in fibroblast-like cells such as hepatic stellate cells and in some neurons. It is thought to consume nitric oxide and protect cells from oxidative stress, which could influence vascular tone. Its physiological roles are still being defined, and it is studied in liver fibrosis and hypoxia biology.
| Target | Cytoglobin (gene CYGB; UniProt Q8WWM9, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 21.4 kDa |
| Observed MW | 21 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat, Mouse) |
|---|
Samples with a confirmed band (WB): rat brain tissue, rat small intestine tissue, rat liver tissue, rat kidney tissue, SGC cells, COLO320 cells, SMMC cells, PANC cells, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the N-terminal region of human cytoglobin. The peptide sequence is conserved in rat. It differs from the mouse sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cytoglobin in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Gomes Bruna R. B. et al. (2019) Cytoglobin Attenuates Neuroinflammation in Lipopolysaccharide-Activated Primary Preoptic Area Cells via NF-κB Pathway Inhibition. Frontiers in Molecular Neuroscience. 10.3389/fnmol.2019.00307
