| Field | Specification |
|---|---|
| Target | |
| Alternative names | Keratin, type I cytoskeletal 14;Cytokeratin-14;CK-14;Keratin-14;K14;KRT14; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Cytokeratin 14 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FEF-11 is a rabbit monoclonal antibody that recognizes keratin, type I cytoskeletal 14 (gene KRT14) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 51.6 kDa, and the supplier reports an observed band at about 52 kDa.
Keratin 14 (KRT14) is a type I intermediate filament protein of the basal, proliferating layer of stratified epithelia. It pairs with keratin 5 to form bundled filaments that anchor to hemidesmosomes and desmosomes and resist mechanical stress. KRT14 is a common basal cell marker, and its mutations cause epidermolysis bullosa simplex, a skin blistering disorder.
| Target | Keratin, type I cytoskeletal 14 (gene KRT14; UniProt P02533, human) |
|---|---|
| Clone | FEF-11 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 51.6 kDa |
| Observed MW | 52 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human A431 cells, human Hacat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human stomach tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HACAT cells.
Immunogen
Synthetic peptide based on the sequence of human cytokeratin 14.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places keratin, type I cytoskeletal 14 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in the corneal epithelium (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zeming Li et al. (2025) Spatiotemporal Adaptations-Driven Dynamic Thra Activation Simulates a Skin Wound Healing Response. Advanced Science. 10.1002/advs.202506651
Zhou Siyi et al. (2025) Contact Cooling-Induced ELOVL4 Enhances Skin Wound Healing by Promoting the Inflammation-to-Proliferation Phase Transition. International Journal of Biological Sciences. 10.7150/ijbs.107871
Jianming Wang et al. (2024) Developing a gel-col heterogeneous network hydrogel scaffold for tissue-engineered skin with enhanced basement membrane formation. Materials Today Communications. 10.1016/j.mtcomm.2024.109219
