| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Keratin, type I cytoskeletal 18;Cytokeratin endo B;Keratin D;Cytokeratin-18;CK-18;Keratin-18;K18;Krt18;Kerd, Krt1-18; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Cytokeratin 18 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-Krt18 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone GBF-11; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-Cytokeratin 18 Krt18 Rabbit Monoclonal Antibody catalog # M01357-2. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: Krt18 (Keratin, type I cytoskeletal 18).
- Antibody format: Monoclonal; clone GBF-11; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
Krt18 (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. . Reported cellular localization context: Nucleus matrix . Nucleus, nucleolus . Cytoplasm . Tissue expression notes (as provided): Expressed in endoderm, intestinal epithelial cells and in most extraembryonic tissues. .
Research relevance and current trends
- Research context keywords from the source record include: Cell Type Markers,Class I,Cytoskeleton,Cytoskeleton/ECM,ECM Proteins,Extracellular Matrix,Intermediate Filaments,Microtubules,Signal Transduction,Structures,Tumor Associated.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate Krt18 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect Krt18 expression by Western blot in cell or tissue lysates, Detect Krt18 in FFPE tissue sections by immunohistochemistry, Localize Krt18 by immunofluorescence/immunocytochemistry in cultured cells, Quantify Krt18-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 48 kDa; calculated MW: 47538 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 48 kDa
- Cellular localization (provided): Nucleus matrix . Nucleus, nucleolus . Cytoplasm .
- Tissue details (provided): Expressed in endoderm, intestinal epithelial cells and in most extraembryonic tissues. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
