| Field | Specification |
|---|---|
| Target | |
| Alternative names | Keratin, type I cytoskeletal 18;Cytokeratin-18;CK-18;Keratin-18;K18;Krt18 ;Krt1-18 ; |
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| Clone | |
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| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines. |
| Molecular weight | |
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Overview
Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90) is an antibody targeting KRT18. Common applications include WB, IHC, IF. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: CY-90; isotype: Mouse IgG1; reactivity: Human; observed MW: 48 kDa; calculated MW: 47761 MW.
Boster Bio Anti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90) catalog # MA1026. Tested in IF, IHC, WB applications. This antibody reacts with Human.
Key elements and design rationale
- Target: KRT18 — Keratin, type I cytoskeletal 18
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: CY-90; Isotype: Mouse IgG1
- Species reactivity: Human
- Molecular weight guidance: Observed: 48 kDa; Calculated: 47761 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection (By similarity). Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. .
Scientific background (datasheet): Intermediate filaments (IFs) are a structurally related family of cellular proteins that appear to be intimately involved with the cytoskeleton. Human keratin 18 (KRT18) and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. The KRT18 gene is 3,791 bp long and the keratin 18 protein is coded for by 7 exons. The K18 gene is 3791 bp in length and the K18 protein is coded for by seven exons. By Southern blotting using the genomic DNA PCR product, the gene for keratin 18 is assigned to chromosome 12. Mutation of human keratin 18 in association with cryptogenic cirrhosis.
Cellular localization (datasheet): Nucleus matrix . Nucleus, nucleolus . Cytoplasm .
Tissue details (datasheet): Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine and in the older intragemmal cells of the tongue. .
Sequence similarities (datasheet): Belongs to the intermediate filament family.
Research relevance and current trends
- Commonly studied in contexts related to Cell Type Markers,Class I,Cytoskeleton,Cytoskeleton/ECM,ECM Proteins,Extracellular Matrix,Intermediate Filaments,Microtubules,Signal Transduction,Structures,Tumor Associated.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
