| Field | Specification |
|---|---|
| Target | |
| Alternative names | Z-DNA-binding protein 1; DNA-dependent activator of IFN-regulatory factors; DAI; Tumor stroma and activated macrophage protein DLM-1; ZBP1; C20orf183; DLM1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human DAI/ZBP1 recombinant protein (Position: A2-E421). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets Z-DNA-binding protein 1 (gene ZBP1) in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 46.3 kDa, and the supplier reports an observed band at about 63 kDa.
ZBP1 (Z-DNA-binding protein 1, also called DAI) is an interferon-induced innate immune sensor that recognizes nucleic acids in the left-handed Z conformation, such as Z-RNA produced during some viral infections. Through its RHIM domain it engages RIPK3 and RIPK1 to trigger necroptosis and other forms of inflammatory cell death, making it a subject of research in antiviral defense and inflammation.
| Target | Z-DNA-binding protein 1 (gene ZBP1; UniProt Q9H171, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 46.3 kDa |
| Observed MW | 63 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HEK293 cells, human HeLa cells, human K562 cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with a confirmed signal (flow cytometry): U87 cells.
Immunogen
Recombinant human DAI/ZBP1 fragment (Ala2–Glu421), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places Z-DNA-binding protein 1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Highly expressed in lymphatic tissues including lymph node, leukocytes, tonsil, bone marrow and spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liu Jie et al. (2026) Suppression of endothelial cell PANoptosis: qingyi decoction targets the TMAO-HMGB1 pathway to mitigate lung injury in acute pancreatitis rats. Apoptosis. 10.1007/s10495-025-02212-y
Haibo Xie et al. (2025) MPTP controls the release of mtDNA and induces endothelial cell PANoptosis in trichloroethylene-induced immune kidney injury. European Journal of Pharmacology. 10.1016/j.ejphar.2025.178118
