| Field | Specification |
|---|---|
| Target | |
| Alternative names | Z-DNA-binding protein 1; DNA-dependent activator of IFN-regulatory factors; DAI; Tumor stroma and activated macrophage protein DLM-1; Zbp1; Dlm1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse DAI/Zbp1 recombinant protein (Position: A2-D382). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets Z-DNA-binding protein 1 (gene Zbp1) in mouse and rat samples and is validated for Western blot, ICC/IF and ELISA. The predicted molecular weight is 44.3 kDa, and the supplier reports an observed band at about 63 kDa.
ZBP1 (Z-DNA-binding protein 1, also called DAI) is an interferon-induced innate immune sensor that recognizes nucleic acids in the left-handed Z conformation, such as Z-RNA produced during some viral infections. Through its RHIM domain it engages RIPK3 and RIPK1 to trigger necroptosis and other forms of inflammatory cell death, making it a subject of research in antiviral defense and inflammation.
| Target | Z-DNA-binding protein 1 (gene Zbp1; UniProt Q9QY24, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 44.3 kDa |
| Observed MW | 63 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat liver tissue, mouse kidney tissue, mouse HEPA1–6 cells, rat thymus tissue, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): RM-1 cells.
Immunogen
Recombinant mouse DAI/Zbp1 fragment (Ala2–Asp382), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places Z-DNA-binding protein 1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (mouse): Expressed in lung, spleen and liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wenchao Wang et al. (2026) mechanical stress-mediated ferritinophagy aggravates cartilage endplate degeneration via Piezo1-NCOA4-ZBP1 axis. Autophagy. 10.1080/15548627.2026.2677183
Yu-qiong He et al. (2023) Ursodeoxycholic acid alleviates sepsis-induced lung injury by blocking PANoptosis via STING pathway. International Immunopharmacology. 10.1016/j.intimp.2023.111161
Zhang Nana et al. (2026) ACT001 alleviates sepsis-induced acute lung injury by downregulating PANoptosis via the JAK2/STAT3 pathway. Inflammation Research. 10.1007/s00011-026-02216-7
Yulei Liang et al. (2024) Shengxian decoction improves lung function in rats with bleomycin-induced idiopathic pulmonary fibrosis through the inhibition of PANoptosis. Journal of Ethnopharmacology. 10.1016/j.jep.2024.118153
Xiongjie Xu et al. (2026) Integrated Multi-Omics Analysis Reveals IRF1-Driven Microglial PANoptosis via ZBP1 in Spinal Cord Injury. Journal of Inflammation Research. 10.2147/JIR.S574990
Xin-yu Peng et al. (2025) PUMA upregulation promotes the necroptosis of hepatocytes in trichloroethylene-sensitized mice via mtDNA-mediated ZBP1 pathway. Toxicology Letters. 10.1016/j.toxlet.2025.111807
