Anti-Danio rerio (Zebrafish) atg5 Antibody

SKU:BHA21012643
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Suppliers
Boster Bio
Boster Bio
Details Products
Overview
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Anti-atg5 antibody (Rabbit polyclonal, Rabbit IgG). Validated for Western blot and immunofluorescence. Reactivity: Zebrafish. Recognizes autophagy protein 5. Supplied in liquid form, ready for use in standard immunodetection workflows.
Target atg5
Host Rabbit
Reactivity Zebrafish
Isotype Rabbit IgG
Application(s) Western blot, IF
Options selector
Catalog no. Size Conjugation
DZ41728 200 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 200 uL/vial
    • Conjugation (12): Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647, Carrier Free

    Variant-specific details:
    • 200 uL/vial + Unconjugated
      Storage: At -20℃ for one year, at 4℃ for one month. Avoid repeated freezing and thawing.
      Form: Liquid
      Applications: Western blot, IF
      Application details: Western blot, 0.25-0.5 μg/ml
      Immunofluorescence, 5 μg/ml
      Contents: Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN237.
    • 200 uL/vial + Biotin; 200 uL/vial + Cy3; 200 uL/vial + Fluoro488; 200 uL/vial + Fluoro550; 200 uL/vial + Fluoro594; 200 uL/vial + FITC; 200 uL/vial + APC; 200 uL/vial + PE; 200 uL/vial + Fluoro647
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
      Form: Liquid
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
    • 200 uL/vial + HRP
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      Form: Liquid
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
    • 200 uL/vial + Carrier Free
      Storage: At -20℃ for one year, at 4℃ for one month. Avoid repeated freezing and thawing.
      Form: Liquid
      Applications: Western blot, IF
      Application details: Western blot, 0.25-0.5 μg/ml
      Immunofluorescence, 5 μg/ml
      Contents: Each vial contains 20mM PBS, 50% glycerol, 0.02% NaN3.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target atg5
Alternative names Autophagy protein 5; atg5; apg5l; zgc:100934
UniProt # F1RDC0
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Zebrafish
Applications
  • Western Blot
  • Immunofluorescence
Conjugate
  • Biotin
  • Cy3
  • Fluoro488
  • Fluoro550
  • Fluoro594
  • FITC
  • HRP
  • APC
  • PE
  • Fluoro647
  • Carrier Free
Purification Immunogen affinity purified.
Cellular localization Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein.
Concentration 500 μg/ml
Form Liquid
Storage At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Catalog no. (Mfr.) DZ41728
Main SKU BHA21012643
Rabbit Polyclonal

Product Overview

This rabbit polyclonal antibody detects autophagy protein 5 (gene atg5) in zebrafish samples and is validated for Western blot and ICC/IF.

ATG5 is a core autophagy protein that is covalently linked to ATG12 by the enzymes ATG7 and ATG10. The ATG12-ATG5 conjugate, bound to ATG16L1, drives LC3 lipidation and autophagosome formation, and ATG5 knockout cells and animals are standard tools for studying autophagy.

Target Autophagy protein 5 (gene atg5; UniProt F1RDC0, zebrafish)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Zebrafish
Form Liquid
Formulation 20 mM PBS; 50% glycerol; 0.02% NaN3
Storage Store at −20 °C for up to 12 months or 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 0.25–0.5 µg/mL
Immunocytochemistry / Immunofluorescence 5 µg/mL

Reactivity Notes

The supplier lists reactivity with zebrafish. UniProt places autophagy protein 5 in the cytoplasm and preautophagosomal structure membrane.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 0.25–0.5 µg/mL; Immunocytochemistry / Immunofluorescence 5 µg/mL. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Zebrafish. Other species are not listed by the supplier.
Q.What are the host species and clonality?
A.Host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG.
Q.How should this antibody be stored?
A.Store at −20 °C for up to 12 months or 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today