| Field | Specification |
|---|---|
| Target | |
| Alternative names | D-amino-acid oxidase; DAAO; DAMOX; DAO |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human DAO recombinant protein (Position: C18-K338). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets D-amino-acid oxidase (gene DAO) in human and mouse samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 39.5 kDa, and the supplier reports an observed band at about 39 kDa.
D-amino acid oxidase (DAO) is a peroxisomal flavoenzyme that breaks down D-amino acids by oxidative deamination, producing keto acids, ammonia and hydrogen peroxide. In the brain it degrades D-serine, a co-agonist of NMDA receptors, so it can influence glutamate signaling. It is studied in neuroscience, schizophrenia research and in kidney handling of dietary D-amino acids.
| Target | D-amino-acid oxidase (gene DAO; UniProt P14920, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 39.5 kDa |
| Observed MW | 39 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human hcct tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): U937 cells.
Immunogen
Recombinant human DAO fragment (Cys18–Lys338), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt describes D-amino-acid oxidase as secreted and also located in the peroxisome matrix, cytoplasm and presynaptic active zone. Tissue expression noted by UniProt (human): Expressed in the cerebellum, in astrocytes of the cortex, in motor neurons and fibers of the lumbar spinal cord (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zehua Niu et al. (2025) The role of DAAO in cognitive impairment of offspring mice induced by arsenic exposure during early developmental stage. PLoS One. 10.1371/journal.pone.0333414
