| Field | Specification |
|---|---|
| Target | |
| Alternative names | TYRO protein tyrosine kinase-binding protein; DNAX-activation protein 12; Killer-activating receptor-associated protein; KAR-associated protein; TYROBP; DAP12; KARAP |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DAP12 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19T88 is a rabbit monoclonal antibody that recognizes TYRO protein tyrosine kinase-binding protein (gene TYROBP) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 12.2 kDa, and the supplier reports an observed band at about 12 kDa.
TYROBP (DAP12) is a transmembrane adaptor that carries an ITAM signaling motif and pairs with activating receptors on NK cells, macrophages, microglia and osteoclasts, such as TREM2. When these receptors bind their ligands, TYROBP recruits SYK to start activation signals, and loss of TYROBP or TREM2 causes Nasu-Hakola disease.
| Target | TYRO protein tyrosine kinase-binding protein (gene TYROBP; UniProt O43914, human) |
|---|---|
| Clone | 19T88 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 12.2 kDa |
| Observed MW | 12 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:80 |
Samples with a confirmed band (WB): human PBMC cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human DAP12.
Reactivity Notes
The supplier lists reactivity with human. UniProt places TYRO protein tyrosine kinase-binding protein in the cell membrane. Tissue expression noted by UniProt (human): Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
