| Field | Specification |
|---|---|
| Target | |
| Alternative names | Atypical chemokine receptor 1; Duffy antigen receptor for chemokines; Duffy antigen/chemokine receptor; Duffy blood group antigen; Fy glycoprotein; GpFy; Glycoprotein D; Plasmodium vivax receptor; CD antigen CD234; ACKR1; DARC; FY; GPD |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DARC |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19A90 is a rabbit monoclonal antibody that recognizes atypical chemokine receptor 1 (gene ACKR1) in human and mouse samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 35.6 kDa, and the supplier reports an observed band at about 45 kDa.
Atypical chemokine receptor 1 (ACKR1, DARC, Duffy antigen) binds many inflammatory chemokines without triggering classical G protein signaling. On red cells and venular endothelium it buffers and transports chemokines, and it is the receptor used by Plasmodium vivax to invade red blood cells.
| Target | Atypical chemokine receptor 1 (gene ACKR1; UniProt Q16570, human) |
|---|---|
| Clone | 19A90 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 35.6 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): human fetal liver.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human DARC.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places atypical chemokine receptor 1 in the early endosome, recycling endosome and membrane. Tissue expression noted by UniProt (human): Found in adult kidney, adult spleen, bone marrow and fetal liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
