Anti-Daxx Rabbit Monoclonal Antibody

SKU:BHA21007841
—
Suppliers
Boster Bio
Boster Bio
Details Products
Overview
Click light‑blue chips for details
Anti-DAXX antibody (Rabbit monoclonal, clone EEI-4, Rabbit IgG). Validated for Western blot, immunohistochemistry, immunofluorescence and flow cytometry. Reactivity: Human. Recognizes death domain-associated protein 6. Observed band in Western blot: about 110 kDa.
Target DAXX
Clone number EEI-4
Host Rabbit
Reactivity Human
Isotype Rabbit IgG
Application(s) WB, IHC, ICC, IF, Flow Cytometry
Options selector
Catalog no. Size Conjugation
M00439 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 uL/vial — Unconjugated
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
      Form: Liquid
      Applications: WB,IHC,ICC,IF,Flow Cytometry
      Application details: WB 1:500-2000
      IHC 1:50-200
      ICC/IF 1:50-200
      FC 1:50
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target DAXX
Alternative names Death domain-associated protein 6;Daxx;hDaxx;ETS1-associated protein 1;EAP1;Fas death domain-associated protein;DAXX;BING2, DAP6;
UniProt # Q9UER7
Host Rabbit
Clone EEI-4
Clonality
  • Monoclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • Flow Cytometry
Immunogen A synthesized peptide derived from human Daxx
Molecular weight 81.4 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Cytoplasm. Nucleus, nucleoplasm. Nucleus, PML body . Nucleus, nucleolus. Chromosome, centromere. Dispersed throughout the nucleoplasm, in PML/POD/ND10 nuclear bodies, and in nucleoli. Colocalizes with histone H3.3, ATRX, HIRA and ASF1A at PML-nuclear bodies. Colocalizes with a subset of interphase centromeres, but is absent from mitotic centromeres. Detected in cytoplasmic punctate structures. Translocates from the nucleus to the cytoplasm upon glucose deprivation or oxidative stress. Colocalizes with RASSF1 in the nucleus. Colocalizes with USP7 in nucleoplasma with accumulation in speckled structures.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M00439
Main SKU BHA21007841
Rabbit Monoclonal

Product Overview

Clone EEI-4 is a rabbit monoclonal antibody that recognizes death domain-associated protein 6 (gene DAXX) in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 81.4 kDa, and the supplier reports an observed band at about 110 kDa.

DAXX (death domain-associated protein 6) is a nuclear protein that often localizes to PML nuclear bodies and acts as a transcriptional corepressor. Together with ATRX it serves as a chaperone that deposits the histone variant H3.3 at telomeres and pericentromeric chromatin, and it also helps control MDM2 and p53 stability. DAXX is studied in chromatin biology, apoptosis and the DNA damage response.

Target Death domain-associated protein 6 (gene DAXX; UniProt Q9UER7, human)
Clone EEI-4
Host / Clonality / Isotype Rabbit / Monoclonal / Rabbit IgG
Reactivity Human
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 81.4 kDa
Observed MW 110 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunohistochemistry 1:50–1:200
Immunocytochemistry / Immunofluorescence 1:50–1:200
Flow cytometry 1:50

Immunogen

Synthetic peptide based on the sequence of human daxx.

Reactivity Notes

The supplier lists reactivity with human. UniProt places death domain-associated protein 6 in the cytoplasm, nucleus and chromosome. Tissue expression noted by UniProt (human): Ubiquitous.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunohistochemistry 1:50–1:200; Immunocytochemistry / Immunofluorescence 1:50–1:200; Flow cytometry 1:50. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 81.4 kDa; observed band at about 110 kDa. A difference from the calculated size is common and can reflect post-translational modification, processing or isoform differences.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human daxx.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Get a Quote

Please use this form for bulk quantity requests or customized products.

Contact Information

Product Information

Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today

Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today