Anti-DAZAP1 Rabbit Monoclonal Antibody

SKU:BHA21010477
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Boster Bio
Boster Bio
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Overview
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Anti-DAZAP1 antibody (Rabbit monoclonal, clone 29D87, IgG). Validated for Western blot. Reactivity: Human, Mouse, Rat. Recognizes DAZ-associated protein 1. Observed band in Western blot: about 43 kDa.
Target DAZAP1
clone number 29D87
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype IgG
Application(s) WB
Options selector
Catalog no. Size Conjugation
M07947 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      • Form: Liquid
      • Applications: WB
      • Application details: WB 1:500-2000
      • Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: Refer to the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: follow the product datasheet storage instructions.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target DAZAP1
Alternative names DAZ-associated protein 1; Deleted in azoospermia-associated protein 1; DAZAP1
UniProt # Q96EP5
Host Rabbit
Clone 29D87
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
Immunogen A synthesized peptide derived from human DAZAP1
Molecular weight 43.4 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Cytoplasm .
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M07947
Main SKU BHA21010477
Rabbit Monoclonal

Product Overview

Clone 29D87 is a rabbit monoclonal antibody that recognizes DAZ-associated protein 1 (gene DAZAP1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 43.4 kDa, and the supplier reports an observed band at about 43 kDa.

DAZAP1 (DAZ-associated protein 1) is an RNA-binding protein with two RRM domains that was first identified as a partner of the germ cell regulators DAZ and DAZL. It is highly expressed in the testis and is thought to contribute to spermatogenesis, and it has also been linked to mRNA processing such as splicing. It is studied in germ cell development and RNA regulation.

Target DAZ-associated protein 1 (gene DAZAP1; UniProt Q96EP5, human)
Clone 29D87
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human, Mouse, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 43.4 kDa
Observed MW 43 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000

Samples with a confirmed band (WB): Jurkat cells.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

Synthetic peptide based on the sequence of human DAZAP1.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. UniProt places DAZ-associated protein 1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Mainly expressed in testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 43.4 kDa; observed band at about 43 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human DAZAP1.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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