| Field | Specification |
|---|---|
| Target | |
| Alternative names | Deleted in azoospermia-like; DAZ homolog; DAZ-like autosomal; Deleted in azoospermia-like 1; SPGY-like-autosomal; DAZL; DAZH; DAZL1; DAZLA; SPGYLA |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DAZL RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACBF-4 is a rabbit monoclonal antibody that recognizes deleted in azoospermia-like (gene DAZL) in human samples and is validated for Western blot. The predicted molecular weight is 33.2 kDa, and the supplier reports an observed band at about 33 kDa.
DAZL (deleted in azoospermia-like) is a germ cell RNA-binding protein needed for the formation of both sperm and eggs. It attaches to the 3′-UTRs of target mRNAs and adjusts how efficiently they are translated, with a particularly central role during spermatogenesis. DAZL is widely used as a germ cell marker and studied in fertility and gametogenesis research.
| Target | Deleted in azoospermia-like (gene DAZL; UniProt Q92904, human) |
|---|---|
| Clone | ACBF-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 33.2 kDa |
| Observed MW | 33 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human testis.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human DAZL RNA-binding protein, which is essential for gametogenesis.
Reactivity Notes
The supplier lists reactivity with human. UniProt places deleted in azoospermia-like in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Testis specific. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
