| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase DCLK1;2.7.11.1;Doublecortin domain-containing protein 3A;Doublecortin-like and CAM kinase-like 1;Doublecortin-like kinase 1;DCLK1;DCAMKL1, DCDC3A, KIAA0369; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DCAMKL1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAFA-4 is a rabbit monoclonal antibody that recognizes serine/threonine-protein kinase DCLK1 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 82.2 kDa, and the supplier reports an observed band at about 82 kDa.
DCLK1 (doublecortin-like kinase 1) is a protein kinase containing doublecortin domains that bind microtubules. In the developing brain it contributes to neuronal migration and dendrite growth, and it also has roles in the mature nervous system. DCLK1 is also used as a marker of tuft cells and certain gastrointestinal stem-like cells, so it is studied in neuroscience and cancer biology.
| Target | Serine/threonine-protein kinase DCLK1 (gene DCLK1; UniProt O15075, human) |
|---|---|
| Clone | AAFA-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 82.2 kDa |
| Observed MW | 82 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:500 |
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human DCAMKL1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): In fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
