| Field | Specification |
|---|---|
| Target | |
| Alternative names | mRNA-decapping enzyme 1A; Smad4-interacting transcriptional co-activator; Transcription factor SMIF; DCP1A; SMIF |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DCP1A Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
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Product Overview
Clone AEFC-4 is a rabbit monoclonal antibody that recognizes mRNA-decapping enzyme 1A (gene DCP1A) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 63.3 kDa, and the supplier reports an observed band at about 75 kDa.
DCP1A is a cofactor that stimulates the decapping enzyme DCP2, which strips the 7-methylguanosine cap from mRNAs and commits them to 5′-to-3′ decay. It is a core component of cytoplasmic P-bodies and takes part in both normal mRNA turnover and nonsense-mediated decay. DCP1A is widely used as a P-body marker in studies of RNA decay and stress granules.
| Target | MRNA-decapping enzyme 1A (gene DCP1A; UniProt Q9NPI6, human) |
|---|---|
| Clone | AEFC-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 63.3 kDa |
| Observed MW | 75 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:100 |
Samples with a confirmed band (WB): 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human DCP1A.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places mRNA-decapping enzyme 1A in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
