| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dynactin subunit 3; Dynactin complex subunit 22 kDa subunit; p22; DCTN3; DCTN22 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DCTN3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24D73 is a rabbit monoclonal antibody that recognizes dynactin subunit 3 (gene DCTN3) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 21.1 kDa, and the supplier reports an observed band at about 21 kDa.
DCTN3 (p24) is a small subunit of dynactin, the multi-protein complex that activates cytoplasmic dynein for processive transport along microtubules. As part of the dynactin shoulder region, it contributes to dynein-driven cargo movement and has been linked to mitotic spindle assembly and cytokinesis. It is studied in intracellular transport and cell division.
| Target | Dynactin subunit 3 (gene DCTN3; UniProt O75935, human) |
|---|---|
| Clone | 24D73 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 21.1 kDa |
| Observed MW | 21 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human DCTN3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places dynactin subunit 3 in the cytoplasm, chromosome, cleavage furrow and midbody. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
