| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | N (G),N (G)-dimethylarginine dimethylaminohydrolase 1;DDAH-1;Dimethylarginine dimethylaminohydrolase 1;3.5.3.18;DDAHI;Dimethylargininase-1;DDAH1;DDAH; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human DDAH1, different from the related mouse and rat sequences by one amino acid. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of DDAH1 (N(G),N(G)-dimethylarginine dimethylaminohydrolase 1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-DDAH1 Antibody Picoband® catalog # PB10000. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human DDAH1, different from the related mouse and rat sequences by one amino acid.
- Molecular weight context: reported MW: 31 kDa, 37 kDa; calculated MW: 31122 MW
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IF, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
N(G),N(G)-dimethylarginine dimethylaminohydrolase 1; N(G),N(G)-dimethylarginine dimethylaminohydrolase 1. DDAH1 is knowns as dimethylarginine dimethylaminohydrolase 1 which is mapped to chromosome 1p22 by radiation hybrid and FISH analysis. This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. DDAH1 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. It widely expressed, especially in liver and kidney. Functional note: Hydrolyzes N (G),N (G)-dimethyl-L-arginine (ADMA) and N (G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. Reported localization: Secreted. Expression/tissue context: Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle. .
Research relevance and current trends
- Cardiovascular: Researchers commonly examine how DDAH1 (N(G),N(G)-dimethylarginine dimethylaminohydrolase 1) relates to this theme using model systems and orthogonal readouts.
- Neuroscience: Researchers commonly examine how DDAH1 (N(G),N(G)-dimethylarginine dimethylaminohydrolase 1) relates to this theme using model systems and orthogonal readouts.
- Neurotransmission: Researchers commonly examine how DDAH1 (N(G),N(G)-dimethylarginine dimethylaminohydrolase 1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative DDAH1 (N(G),N(G)-dimethylarginine dimethylaminohydrolase 1) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of DDAH1 (N(G),N(G)-dimethylarginine dimethylaminohydrolase 1) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
