| Field | Specification |
|---|---|
| Target | |
| Alternative names | N; G; ,N; -dimethylarginine dimethylaminohydrolase 1; DDAH-1; Dimethylarginine dimethylaminohydrolase 1; DDAHI; Dimethylargininase-1; DDAH1; DDAH |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DDAH1 Hydrolyzes N (G),N (G) -dimethyl-L-arginine (ADMA) and N (G) -monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEAG-4 is a rabbit monoclonal antibody that recognizes N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 (gene DDAH1) in human, monkey, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 31.1 kDa, and the supplier reports an observed band at about 37 kDa.
DDAH1 (dimethylarginine dimethylaminohydrolase 1) breaks down asymmetric dimethylarginine (ADMA) and monomethylarginine, endogenous inhibitors of nitric oxide synthase. By clearing these inhibitors, it supports nitric oxide production. DDAH1 is studied in vascular biology, endothelial function and cardiovascular research.
| Target | N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 (gene DDAH1; UniProt O94760, human) |
|---|---|
| Clone | AEAG-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 31.1 kDa |
| Observed MW | 37 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:40 |
| Flow cytometry | 1:30 |
Samples with a confirmed band (WB): human 293T cells, human HeLa cells, monkey COS-7 cells, human SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human DDAH1 Hydrolyzes N (G),N (G) -dimethyl-L-arginine (ADMA) and N (G) -monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. Tissue expression noted by UniProt (human): Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
