| Field | Specification |
|---|---|
| Target | |
| Alternative names | Putative hydrolase DDAH2; DDAHII; Inactive N(G),N(G)-dimethylarginine dimethylaminohydrolase 2; DDAH-2; Inactive dimethylarginine dimethylaminohydrolase 2; Protein G6a; S-phase protein; DDAH2; DDAH; G6A; NG30 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DDAH2 Hydrolyzes N (G),N (G) -dimethyl-L-arginine (ADMA) and N (G) -monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEGG-4 is a rabbit monoclonal antibody that recognizes putative hydrolase DDAH2 in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 29.6 kDa, and the supplier reports an observed band at about 30 kDa.
DDAH2 is a protein related to the ADMA-degrading enzyme DDAH1, but its own enzymatic substrate is not known and it does not appear to hydrolyze ADMA. Studies in endothelial cells suggest it can promote VEGF expression through a nitric oxide-independent route. DDAH2 is studied in vascular and immune cell biology, though its function is not fully characterized.
| Target | Putative hydrolase DDAH2 (gene DDAH2; UniProt O95865, human) |
|---|---|
| Clone | AEGG-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 29.6 kDa |
| Observed MW | 30 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human DDAH2 Hydrolyzes N (G),N (G) -dimethyl-L-arginine (ADMA) and N (G) -monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places putative hydrolase DDAH2 in the cytoplasm and mitochondrion. Tissue expression noted by UniProt (human): Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
