| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA damage-binding protein 1;DDB p127 subunit;DNA damage-binding protein a;DDBa;Damage-specific DNA-binding protein 1;HBV X-associated protein 1;XAP-1;UV-damaged DNA-binding factor;UV-damaged DNA-binding protein 1;UV-DDB 1;XPE-binding factor;XPE-BF;Xeroderma pigmentosum group E-complementing protein;XPCe;DDB1;XAP1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human DDB1 recombinant protein (Position: S1011-H1140). Human DDB1 shares 99.2% amino acid (aa) sequence identity with both mouse and rat DDB1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes DNA damage-binding protein 1 (gene DDB1) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 127.0 kDa, and the supplier reports an observed band at about 127 kDa.
DDB1 (DNA damage-binding protein 1) is a large adaptor with dual roles. With DDB2 it forms the UV-DDB complex that recognizes UV-induced DNA lesions and starts global genome nucleotide excision repair, and it links many substrate receptors (DCAFs) to CUL4 ubiquitin ligases. DDB1 is studied in DNA repair, protein degradation and targeted protein degradation drug research.
| Target | DNA damage-binding protein 1 (gene DDB1; UniProt Q16531, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 127.0 kDa |
| Observed MW | 127 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| IHC (frozen sections) | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 0.5–1 µg/mL |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, human HEK293 cells, human K562 cells, human Raji cells, human Caco-2 cells, human THP-1 cells, rat brain tissue, rat kidney tissue, rat C6 cells, mouse brain tissue, mouse kidney tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse liver tissue, rat liver tissue, human intestinal cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (IHC): human placenta tissue.
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with confirmed staining (ICC/IF): A549 cells, PC-3 cells, SMMC-7721 cells.
Samples with a confirmed signal (flow cytometry): 293T cells.
Immunogen
Recombinant human DDB1 fragment (Ser1011–His1140), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 99.2%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA damage-binding protein 1 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
