Anti-DDB1 Monoclonal Antibody

Overview

This product is an anti-DDB1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AFAH-4; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, IP (as provided in the source record). Boster Bio Anti-DDB1 Monoclonal Antibody catalog # M00333. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: DDB1 (Cytochrome b-245 heavy chain).
• Antibody format: Monoclonal; clone AFAH-4; isotype Rabbit IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

DDB1 (protein: P2X purinoceptor 1) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H (+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Reported cellular localization context: Cell membrane; Multi-pass membrane protein. Tissue expression notes (as provided): Detected in neutrophils (at protein level). .

Research relevance and current trends

• Research context keywords from the source record include: DNA/RNA,DNA Damage & Repair,DNA Damage Response,Epigenetics and Nuclear Signaling.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
• Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.

Workflow ideas (metafield): Validate DDB1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect DDB1 expression by Western blot in cell or tissue lysates, Detect DDB1 in FFPE tissue sections by immunohistochemistry, Localize DDB1 by immunofluorescence/immunocytochemistry in cultured cells, Enrich DDB1 by immunoprecipitation from lysates for downstream analysis

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 47 kDa; calculated MW: 65336 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 47 kDa
• Cellular localization (provided): Cell membrane; Multi-pass membrane protein.
• Tissue details (provided): Detected in neutrophils (at protein level). .

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.