| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA damage-binding protein 2;DDB p48 subunit;DDBb;Damage-specific DNA-binding protein 2;UV-damaged DNA-binding protein 2;UV-DDB 2;DDB2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human DDB2 recombinant protein (Position: M1-T115). Human DDB2 shares 58.3% amino acid (aa) sequence identity with mouse DDB2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects DNA damage-binding protein 2 (gene DDB2) in human samples and is validated for flow cytometry, ICC/IF and Western blot. The predicted molecular weight is 47.9 kDa, and the supplier reports an observed band at about 48 kDa.
DDB2 (also called XPE) is the DNA damage-recognition subunit of the UV-DDB complex. It binds UV-induced lesions such as cyclobutane pyrimidine dimers and 6-4 photoproducts and, with DDB1 and CUL4, promotes their repair through global genome nucleotide excision repair. Mutations cause xeroderma pigmentosum group E, so DDB2 is studied in DNA repair and UV damage research.
| Target | DNA damage-binding protein 2 (gene DDB2; UniProt Q92466, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 47.9 kDa |
| Observed MW | 48 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): A431 cells, SW620 cells, HeLa cells, Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 40 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): 293T cells.
Immunogen
Recombinant human DDB2 fragment (Met1–Thr115), expressed in E. coli. Sequence identity with the mouse ortholog: 58.3%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places DNA damage-binding protein 2 in the nucleus and chromosome. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
