| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA damage-inducible transcript 3 protein;DDIT-3;C/EBP zeta;C/EBP-homologous protein;CHOP;C/EBP-homologous protein 10;CHOP-10;CCAAT/enhancer-binding protein homologous protein;Growth arrest and DNA damage-inducible protein GADD153;DDIT3;CHOP, CHOP10, GADD153; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DDIT3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOHF-4 is a rabbit monoclonal antibody that recognizes DNA damage-inducible transcript 3 protein (gene DDIT3) in human, mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 19.2 kDa, and the supplier reports an observed band at about 29 kDa.
DDIT3 (CHOP, also called GADD153) is a transcription factor of the C/EBP family that is strongly induced by endoplasmic reticulum stress through the unfolded protein response. It can inhibit other C/EBP proteins while activating stress-response genes, and prolonged induction promotes cell cycle arrest and apoptosis. CHOP is a widely used marker of ER stress.
| Target | DNA damage-inducible transcript 3 protein (gene DDIT3; UniProt P35638, human) |
|---|---|
| Clone | AOHF-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 19.2 kDa |
| Observed MW | 29 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells, human U-87MG cells, human U251 cells, rat C6 cells, rat PC-12 cells, mouse NIH/3T3 cells, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human DDIT3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA damage-inducible transcript 3 protein in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Mina Sadat Izadi et al. (2022) Oxidative and endoplasmic reticulum stress develop adverse metabolic effects due to the high-fat high-fructose diet consumption from birth to young adulthood. Life Sciences. 10.1016/j.lfs.2022.120924
Cixia Li et al. (2023) Ketogenic diet attenuates cognitive dysfunctions induced by hypoglycemia via inhibiting endoplasmic reticulum stress-dependent pathways. Food & Function. 10.1039/D3FO04007K
Guo Yazhou et al. (2021) CHOP Regulates Endoplasmic Reticulum Stress-Mediated Hepatoxicity Induced by Monocrotaline. Frontiers in Pharmacology. 10.3389/fphar.2021.685895
Yuzhen Sui et al. (2024) BHBA attenuates endoplasmic reticulum stress-dependent neuroinflammation via the gut–brain axis in a mouse model of heat stress. CNS Neuroscience & Therapeutics. 10.1111/cns.14840
Mine Caglayan et al. (2023) Potential impacts of bisphenols on prostate cells: An overview of cytotoxicity, proliferation, oxidative stress, apoptosis, and ER-stress response activation. Food and Chemical Toxicology. 10.1016/j.fct.2023.114416
Xiang Xuelian et al. (2024) Liproxstatin-1 attenuates acute hypertriglyceridemic pancreatitis through inhibiting ferroptosis in rats. Scientific Reports. 10.1038/s41598-024-60159-7
Fateme Binayi et al. (2021) The chemical chaperon 4-phenyl butyric acid restored high-fat diet- induced hippocampal insulin content and insulin receptor level reduction along with spatial learning and memory deficits in male rats. Physiology & Behavior. 10.1016/j.physbeh.2021.113312
Congcheng Zhang et al. (2026) Retrorsine-induced hepatotoxicity is mediated by inhibition of the EGFR/AKT/c-Jun axis and disruption of calcium homeostasis in primary hepatocytes. Toxicon. 10.1016/j.toxicon.2026.108998
Yanping Cao et al. (2014) Role of endoplasmic reticulum stress in apoptosis of differentiated mouse podocytes induced by high glucose. International Journal of Molecular Medicine. 10.3892/ijmm.2014.1642
Cailian Wu et al. (2019) Effect of endoplasmic reticulum stress involved in manganese‑induced neurotoxicity in rats. Molecular Medicine Reports. 10.3892/mmr.2019.10175
