| Field | Specification |
|---|---|
| Target | |
| Alternative names | Discoidin domain-containing receptor 2; Discoidin domain receptor 2; CD167 antigen-like family member B; Discoidin domain-containing receptor tyrosine kinase 2; Neurotrophic tyrosine kinase, receptor-related 3; Receptor protein-tyrosine kinase TKT; Tyrosine-protein kinase TYRO10; CD antigen CD167b; DDR2; NTRKR3; TKT; TYRO10 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DDR2. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACEB-4 is a rabbit monoclonal antibody that recognizes discoidin domain-containing receptor 2 (gene DDR2) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 96.7 kDa, and the supplier reports an observed band at about 100 kDa.
DDR2 (discoidin domain receptor 2) is a receptor tyrosine kinase activated by fibrillar collagens and is mainly found on mesenchymal cells such as fibroblasts. It regulates extracellular matrix remodeling, cell migration and proliferation, and it is required for normal bone growth through effects on osteoblasts and chondrocytes. DDR2 is studied in skeletal development, fibrosis and cancer.
| Target | Discoidin domain-containing receptor 2 (gene DDR2; UniProt Q16832, human) |
|---|---|
| Clone | ACEB-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 96.7 kDa |
| Observed MW | 100 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human SH-SY5Y cells, human U251 cells, human U2OS cells, rat C6 cells, rat PC-12 cells, mouse RAW264.7 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human DDR2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places discoidin domain-containing receptor 2 in the cell membrane. Tissue expression noted by UniProt (human): Detected in osteocytes, osteoblastic cells in subchondral bone, bone lining cells, tibia and cartilage (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yinghua Liu et al. (2026) Dissecting Discoidin Domain Receptor 2 Dynamics in Fibrosis with a Programmable Collagen-Mimetic Probe. ACS Nano. 10.1021/acsnano.6c04093
