| Field | Specification |
|---|---|
| Target | |
| Alternative names | ATP-dependent RNA helicase DDX1; DEAD box protein 1; DEAD box protein retinoblastoma; DBP-RB; DDX1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human DDX1 recombinant protein (Position: K562-F740). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets ATP-dependent RNA helicase DDX1 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry, ELISA and immunoprecipitation. The predicted molecular weight is 82.4 kDa, and the supplier reports an observed band at about 88–90 kDa.
DDX1 is an ATP-dependent RNA helicase of the DEAD-box family that can unwind RNA-RNA and RNA-DNA duplexes. It takes part in several RNA processes, including tRNA splicing as part of the tRNA ligase complex, and has been linked to RNA clearance at DNA double-strand breaks. DDX1 is studied in RNA metabolism, DNA repair and viral replication.
| Target | ATP-dependent RNA helicase DDX1 (gene DDX1; UniProt Q92499, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 82.4 kDa |
| Observed MW | 88–90 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human K562 cells, human Caco-2 cells, human SH-SY5Y cells, human RT4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat testis tissue, mouse testis tissue, mouse ovary tissue, human colon tissue, mouse brain tissue, rat brain tissue, human colon cancer tissue, human lung cancer tissue, human ovarian cancer tissue, human pancreas cancer tissue, human skin cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with confirmed staining (ICC/IF): human pancreas cancer tissue, human skin cancer tissue.
Samples with a confirmed result (IP): SH-SY5Y cells.
Samples with a confirmed signal (flow cytometry): SiHa cells.
Immunogen
Recombinant human DDX1 fragment (Lys562–Phe740), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ATP-dependent RNA helicase DDX1 in the nucleus, cytoplasm, cytoplasmic granule and mitochondrion. Tissue expression noted by UniProt (human): Highest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
