| Field | Specification |
|---|---|
| Target | |
| Alternative names | Probable ATP-dependent RNA helicase DDX5; DEAD box protein 5; RNA helicase p68; DDX5; G17P1; HELR; HLR1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human DDX5 RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADBC-4 is a rabbit monoclonal antibody that recognizes probable ATP-dependent RNA helicase DDX5 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 69.1 kDa, and the supplier reports an observed band at about 54 kDa.
DDX5 (p68) is a prototype DEAD-box RNA helicase that uses ATP to remodel RNA structure. It takes part in pre-mRNA and alternative splicing, microRNA processing and ribosome biogenesis, and acts as a transcriptional coregulator for factors such as the androgen receptor and p53. DDX5 is studied in gene expression control, differentiation and cancer.
| Target | Probable ATP-dependent RNA helicase DDX5 (gene DDX5; UniProt P17844, human) |
|---|---|
| Clone | ADBC-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 69.1 kDa |
| Observed MW | 54 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, human SH-SY5Y cells, rat heart tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue, mouse cerebellum tissue, rat cerebellum tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human DDX5 RNA-dependent ATPase activity.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places probable ATP-dependent RNA helicase DDX5 in the nucleus, nucleus speckle and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yanchun Zhou et al. (2024) SNHG15 Mediates MTSS1 Gene Expression via Interacting with the Gene Promoter and Regulating Transcription Pausing. International Journal of Molecular Sciences. 10.3390/ijms252111565
